Human Primary Cells
Human Follicle Outer Root Sheath Cells (HORSC)
Human Follicle Outer Root Sheath Cells (HORSC) are primary cells isolated from the outer root sheath of the hair follicle and are cryopreserved at third passage. HORSC can be propagated at least 4 doublings. Outer root sheath cells can be cultured in vitro to form epidermal equivalents1, and can be used for autologous grafting2. Furthermore, HORSC are reported to support stem cells in the bulge region of the outer sheath3. These bulge cells are mutlipotent, critical for the self-renewal of hair follicles3, and also have the capacity to regenerate sebaceous glands and epidermis4.  

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name

Product Description

Size (vol)

Cat No.
HORSC Total Kit Each kit contains an ampoule of cryopreserved HORSC (#606-05a-05), 500 ml of Outer Root Sheath Growth Medium (617-500), and a Subculture Reagent Kit (090K). 1 kit 095606-05a
 
Cryopreserved HORSC, adult (>500,000 cells in an ampoule) Cryopreserved in ampoules at second passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 ampoule 095606-05a
 
Outer Root Sheath Growth Medium Optimized and ready for use. 500 ml 095617-500
 
Outer Root Sheath Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500ml 095617K-500
 
Outer Root Sheath Basal Medium Add Growth Supplements before use. 500 ml 095616-500
 
Outer Root Sheath Growth Supplements   30 ml 095617-GS
 
Subculture Reagent Kit (100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution) 100 ml 095090K
 
  1. Lenoir, MC et al., Dev Viol. 130(2):610 (1988).
  2. Limat, A & Hunziker, T. Cells Tissues Organs. 172(2):79 (2002).
  3. Ohyama, M et al., J Cell Invest. 116(1):19 (2006).
  4. Ohyama, M, J Dermatol Sci. (2007).
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