Human Primary Cells
Hair Follicle Dermal Papilla Cells (HFDPC)

 

Human Hair Follicle Dermal Papilla Cells (HFDPC) are mesenchyme cells isolated from the hair papilla of normal human scalp hair follicles. They are cryopreserved at second passage, and can be cultured and propagated 6 population doublings. HFDPC can be used for the development and evaluation of hair growth products. Hair papilla, in the adult hair follicle, plays a crucial role in the dermal-epidermal interactions that control hair production and events of the hair growth cycle. It has been shown that cultured HFDPC can stimulate hair growth when implanted into an amputated follicle1. When used in a reconstitution model, HFDPC is an excellent tool for identifying cell populations within the hair follicle and studying the effects of specific gene products on hair follicle growth and development2.

  
Characterization: Positive for alkaline phosphatase. Negative for vWF antigen expression.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol)

Cat. No.
HFDPC Total Kit , adult

Each kit contains an ampoule of cryopreserved HFDPC (602-05a), 500 ml of HFDPC Growth Medium (611-500), a Subculture Reagent Kit, and 50 ml of Collagen Coating Solution (125-50).

 1 Kit 602K-05a
Cryopreserved HFDPC , adult (>500,000 cells in an ampoule) Cryopreserved at third passage in Papilla Cell Basal Medium that contains 20% FBS and 5% DMSO. 1 Ampoule 602-05a
HFDPC Growth Medium

Optimized and ready for use

 500 ml 611-500
HFDPC Growth Medium Kit Basal Medium & Growth Supplement packaged separately

Yields 500 ml

 611K-500
HFDPC Basal Medium Add Growth Supplement before use 500 ml 610-500
HFDPC Growth Supplement   30 ml 611-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K
Collagen Coating Solution   50 ml 125-50
HFDPC RNA , adult

Total RNA prepared from HFDPC

10 µg

25 µg

602-R10a

602-R25a
 
  1. Jahoda, C. et al, J. Invest. Dermatol. 101:584 (1993).
  2. Weinberg, W. et al, J. Invest. Dermatol. 100: 229 (1993).
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