Human Primary Cells
Human Preadipocytes (HPAd)

Human Preadipocytes (HPAd) are derived from human adipose tissue. These fibroblast-like precursor cells are cryopreserved at the end of primary culture and can be propagated two passages prior to differentiating into Human Adipocytes (HAd). HPAd derived HAd were used to study insulin-stimulated glucose transport1,2, growth hormone enhanced lypolysis3 and obese gene expression4. HPAd can be fully differentiated into mature HAd in CAIµs Adipocyte Differentiation Medium. Significant differences in lipolysis and leptin production4 are observed in HPAd and HAd from human omental and subcutaneous adipose tissue. We provide HPAd from subcutaneous adipose tissue at various sites and adipose depot on the heart. HPAd/HAd are a useful in vitro cellular model for investigating the causes and treatment of obesity, type II diabetes and cardiovascular diseases. Recently it was discovered that human adipocytes might be a new target for the immunodeficiency virus-15.

  

Characterization: Positive oil droplet accumulation.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Subcutaneous

Product Name

 Product Description Size (vol) Cat. No.
Preadipocytes Human

Subcutaneous, BMI<24,99, cryopreserved

 2E6 cells 088SP-F-1
Preadipocytes Human Subcutaneous, 24,99<29,99, cryopreserved 2E6 cells 088SP-F-2
Preadipocytes Human Subcutaneous, BMI>30, cryopreserved 2E6 cells 088SP-F-3
Preadipocytes Human Subcutaneous, Superlot (pooled with average BMI), cryopreserved 2E6 cells 088SP-F-SL
Preadipocytes Human Subcutaneous, from diabetic donor, BMI>30, cryopreserved 1E6 cells 088SPD-F-3
Preadipocytes Human Facial Subcutaneous facial (subcutaneous eye), BMI: varies, cryopreserved 1E6 cells 088SQE-F
Preadipocytes Breast Cells Subcutaneous breast, BMI: varies, cryopreserved 1E6 cells 088BR-F

Visceral

Product Name

 Product Description Size (vol) Cat. No.
Preadipocyte Human Omental

Omental, BMI>30, cryopreserved

 1E6 cells 088OP-F-3
Preadipocyte Human Omental

Omental human, from a diabetic donor, BMI>30, cryopreserved

 1E6 cells 088OPD-F

Preadipocyte Human Matched Set

 Subcutaneous and Omental cells (1 vial 1E6 cells each), BMI>30, cryopreserved 1E6 cells 088MS-F-3
Preadipocyes Human Reri-Renal Peri-Renal, BMI: varies, cryopreserved 1E6 cells 088PR-F
Preadipocytes Human Mesenteric Mesenteric, BMI: varies, cryopreserved 1E6 cells 088MSN-F

Heart

Product Name

 Product Description Size (vol) Cat. No.

HPAd Total Kit adult

heart (h)

Each kit contains an ampoule of cryopreserved HPAd (802-05), 500 ml of Preadipocyte Growth Medium (811-500), and a Subculture Reagent Kit.

 1 Kit 095802hK-05ha

Cryopreserved HPAd adult

heart (h)

(>500,000 cells in an ampoule) Cryopreserved at second passage in Preadipocyte Cell Basal Medium that contains 10% FBS and 10% DMSO.

 1 Ampoule 095802h-05a

Medium for Subcutaneous cells

Product Name

 Product Description Cat. No.
Adipocyte Medium   088AM-1
Adipocyte Medium PRF Phenol Red Free 088AM-1-PRF
Adipocyte Medium PRF-SF
 Phenol Red Free / Serum Free 088AM-1-PRF-SF
Adipocyte Medium Serum Free Serum Free 088AM-1-SF
Differenciation Medium   088DM-2/10
Differenciation Medium PRF Phenol Red Free 088DM-2/10-PRF
Differenciation Medium Serum Free   088DM-2/10-SF
Preadipocyte Medium   088PM-1
Preadipocyte Medium PRF Phenol Red Free 088PM-1-PRF
Preadipocyte Medium PRF-SF Phenol Red/Serum Free 088PM-1-PRF-SF
Preadipocyte Medium SF Serum Free 088PM-1-SF

Medium for Omental cells

Product Name

 Product Description Cat. No.
Omental Preadipocyte Medium   088OM-PM
Omental Adipocyte Differentiation Medium   088OM-DM
Omental Adipocyte Maintenance Medium   088OM-AM
Omental Adipocyte Basal Medium   088OM-BM

Medium for Heart cells

Product Name

 Product Description Cat. No.
Preadipocyte Growth Medium Optimized & ready for use 095811-500
Preadipocyte Growth Medium Kit Basal Medium & Growth Supplement packaged separately 095811K-500
Preadipocyte Basal Medium Add Growth Supplement before use 095810-500
Preadipocyte Growth Supplement   095811-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 095090K
HPAd RNA subcutaneous(s) Total RNA prepared from HPAd 095802s-R10a

095802s-R25a
  1. Tanti, J-F. et al., Endocrinology 138(5):2005 (1997)
  2. Okada, T. et al., J. Biochem Mol Biol 269(5):3568 (1994)
  3. Nam, S.Y. et al., Obes Rev 1(2):73 (2000)
  4. Hamilton, B.S. et al, Nature Medicine 1(9):953 (1995)
  5. van Harlemen, V. et al., Diabetes 51:2029 (2002)
  6. Hazan, U. et al., FASEB J. 16(10):1254 (2002)
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