Human Primary Cells
Human Adipocytes (HAd)

 

Previously, most research in adipocyte biology was conducted in mouse 3T3-L1 cell lines1,2,3. It was impossible to use human adipocytes because they die within 24 hours after isolation. To facilitate the study of human adipocyte biology, now we provide HAd (Human Adipocytes) which were differentiated from HPAd (Human Preadipocytes) through use of Adipocyte Differentiation Medium. Cells are shipped 10 days after initiating differentiation. Mature HAd are expected 15 days after induction of differentiation and should remain healthy and responsive for at least 2 weeks after complete differentiation to mature HAd. Adipose mass can be controlled by inhibition of HPAd differentiation and increase of lypolysis. HAd are ideal cellular models for drug discovery research in the area of obesity and diabetes.   Human Adipocytes, HAd HAd

Lipid stained human adipocytes, HAd

Oil Red O stained lipid accumulation in HAd
Characterization: Positive for lipid accumulation on HAd.

Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, and negative for mycoplasma, bacteria, yeast and fungi.

Product Name

Product Description

Size (vol)

Cat. No.
Plated adipocytes Custom services Plates 101SCC_01
Adipocyte Differentiation Medium   250 ml 095811D-250
Adipocyte Maintenance Medium   250 ml 095811M-250
Lysis Buffer   5 ml 095030-005
HAd RNA, adult subcutaneous(s)

Total RNA prepared from HAd.

10 µg

25 µg

 095802sD-R10a

095802sD-R25a
 

  1. Russel, T. R. et al, Proc. Natl. Acad. Sci. USA 73:4516 (1976)

  2. Schmidt, W. et al, J. Biol. Chem. 265:15489 (1990)

  3. Spiegelman, B. M. et al, Cell 24:503 (1981)

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