Human Primary Cells
Human Subclavian Artery Endothelial Cells (HScAEC)

Human Subclavian Artery Endothelial Cells (HScAEC ) are derived from normal human subclavian arteries. They are cryopreserved at second passage and can be cultured and propagated at least 10 population doublings.

The shape and orientation of HScAEC was found to be strongly affected by blood flow conditions1. Autologous leukocyte adhesion to normal and pathologically altered subclavian arteries ex vivo was explored2. The effect of prolonged treatment with r-hirudin after angioplasty of the subclavian artery was evaluated in rabbits3, whereas rabbit ScAEC was used to study the effects of dietary and genetically induced hypercholesterolaemia on the vasodilator and antiaggregatory capacity of the endothelium 4.

  

Subclavian Artery Endothelial Cells

Characterization: Positive for DiI-Ac-LDL uptake. Positive for factor VIII-related antigen expression.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol)

Cat. No.
HScAEC Total Kit , adult

Each kit contains an ampoule of cryopreserved HScAEC (3010-05a), 500 ml of HScAEC Growth Medium (212-500), and a Subculture Reagent Kit (090K).

1 Kit

 0953010K-05a
 

Cryopreserved HScAEC, adult

 (>500,000 cells in an ampoule) Cryopreserved at second passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 Ampoule

 0953010-05a
 

HScAEC Growth Medium

Optimized and ready for use

500 ml

 095212-500
 
HScAEC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095212K-500
 
HScAEC Basal Medium Add Growth Supplement before use 500 ml 095210-500
 
HScAEC Growth Supplement   30 ml 095212-GS
 
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090K
 

HScAEC RNA, adult

Total RNA prepared from HScAEC

10 µg

 0953010-R10a
 

HScAEC RNA, adult

Total RNA prepared from HScAEC

25 µg

 0953010-R25a
 
 
  1. Sato, M. and Ohshima, N. Biorheology 31(2):143-53 (1994).
  2. Kennedy, S. et al, J. Immunol. Methods 244(1-2):153-62 (2000).
  3. Hadoke, P.W. et al, Coron. Artery Dis. 7(8):599-608 (1996).
  4. Greenlees, C. et al, Br. J. Pharmacol. 119(7):1470-6 (1996).
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