Human Primary Cells
Human Internal Thoracic (Mammary) Artery Smooth Muscle Cells (HITASMC)

Human intrathoratic artery smooth muscle cells (HITASMC) are derived from the intrathoratic artery. They are cryopreserved at second passage. HITASMC can be cultured and propagated 8 population doublings. Proliferation, migration and invasion of SMC are essential pathogenic processes in the development of a broad spectrum of cardiovascular disorders. A Boyden chamber was employed to characterize the diverging migratory and invasion potential of proliferating and nonproliferating human artery SMCs. The differential gene expression of both phenotypes was analyzed by a cDNA array system. Several candidate genes were identified1. Left internal mammary arteries synthesize endothelium-derived hyperpolarizing factor (EDHF), a short-lived K(+) channel activator that persists after inhibition of nitric oxide (NO) and prostaglandin synthesis. The identity of EDHF is identified as 11,12-epoxyeicosatrienoic acid (11,12-EET) produced by an EDHF synthase CYP450-2C, and accounting for about 40% net endothelial cell relaxation2. Expression of non-degradable IkappaB-alpha might have therapeutic potential in both vascular inflammatory reaction and SMC proliferation3.Using HITSMC, it was found that amolodipine can alter Ca2+ mobilization and inhibit voltage-dependent Ca2+ influx and the resulting ERK 1/2 activation4. In human vascular smooth muscle cells of both saphenous vein and internal thoracic artery, UTP was found to act as an anti-proliferative regulator5.   Human Internal Thoracic Artery Smooth Muscle Cells, HITASMC

HITASMC

Characterization: Positive for smooth muscle cell specific a -actin expression.
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol)

Cat. No.
HITASMC Total Kit adult

Each kit contains an ampoule of cryopreserved HITASMC (358-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit.

 1 Kit 095358K-05a
 
Cryopreserved HITASMC , adult (>500,000 cells in an ampoule) Cryopreserved at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095358-05a
 
Smooth Muscle Cell Growth Medium

Optimized and ready for use .

 500 ml 095311-500
 
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095311K-500
 
Smooth Muscle Cell Basal Medium Add Growth Supplement before use 500 ml 095310-500
 
Smooth Muscle Cell Growth Supplement   30 ml 095311-GS
 
Smooth Muscle Cell Differentiation Medium   250 ml 095311D-250
 
Subculture Reagent Kit (100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution) 100 ml 095090K
 
HITASMC RNA , adult

Total RNA prepared from HITASMC

10 µg

25 µg

 095358-R10a

095358-R25a
 
 
  1. Blindt R, et al. J. Vasc. Res. 2002 Jul; 39(4): 340-52.
  2. Archer SL, et al. Circulation. 2003 Feb 11; 107(5): 769-76.
  3. Wang Z, et al. J Surg. Res. 2002 Feb; 102(2):198-206.
  4. Stepien O, et al. J. Hypertens.2002 Jan; 20(1):95-102.
  5. White PJ, et al. Am J Physiol Heart Circ Physiol. 2000 Dec; 279(6): H2735-42.
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