Human Primary Cells
Human Internal Thoracic (Mammary) Artery Endothelial Cells (HITAEC)

Human Internal Thoracic (Mammary) Artery Endothelial Cells (HITAEC) are derived from normal human internal thoracic arteries. They are cryopreserved at second passage and can be cultured and propagated at least 10 population doublings.

Study showed that the loss of endothelium-dependent relaxation could dangerously predispose the ITA graft to vasospasm in the post-operative period1. Increased incidence of VCAM-1 and ICAM-1 in the aorta and ITA may partly explain the enhanced atherosclerosis associated with diabetes mellitus2. Contractile responses to angiotensin I and II in the radial artery and internal thoracic artery (ITA) segments were examined in vitro3. Acetylcholine induces endothelium-dependent relaxation of the perforating branch of the ITA by stimulation of mucarinic receptors on the endothelial cells4.

  

Internal Thoracic Artery Endothelial Cells, HITAEC

Characterization: Positive for factor VIII-related antigen expression. Positive for Dil-Ac-LDL uptake.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol)

Cat. No.
HITAEC Total Kit , adult

Each kit contains an ampoule of cryopreserved HITAEC (308-05a), 500 ml of HITAEC Growth Medium (212-500), and a Subculture Reagent Kit (090k).

 1 Kit 095308K-05a
 
Cryopreserved HITAEC , adult (>500,000 cells in an ampoule) Cryopreserved at second passage in Endothelial Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095308-05a
 
HITAEC Growth Medium

Optimized and ready for use

 500 ml 095212-500
 
HITAEC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095212K-500
 
HITAEC Basal Medium Add Growth Supplement before use 500 ml 095210-500
 
HITAEC Growth Supplement   30 ml 095212-GS
 
Subculture Reagent Kit

100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution

 100 ml 095090K
 
HITAEC RNA , adult

Total RNA prepared from HITAEC

 10 µg 095308-R10a
 
HITAEC RNA , adult

Total RNA prepared from HITAEC

 25 µg 095308-R25a
 
 
  1. Schyvens, C.G. et al, Clin. Exp. Pharmacol. Physiol. 24(3-4):223-8 (1997).
  2. Ribau, J.C. et al, Surg. Res. 85(2):225:33 (1999).
  3. Borland, J.A. et al, Ann. Thorac. Surg. 70(6):2054-63 (2000).
  4. Pesic, S. et al, Pharmacology 63(3):185-90 (2001).
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