Human Primary Cells
Human Pulmonary Artery Smooth Muscle Cells (HPASMC)

Human Pulmonary Artery Smooth Muscle Cells (HPASMC) are derived from tunica intima and tunica media of normal human, fibrous plaque-free pulmonary arteries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. HPASMC are well suited for the study of pulmonary vascular disorders1, associated liver diseases2, and connective tissue diseases3. Phenotypic changes of smooth muscle cells have been found to be related to smooth muscle cell migration and intimal proliferation. Switches in smooth muscle cell phenotype lead to pulmonary hypertension and eventually heart and lung disorders4.

   HPASMC a-actin stain
Characterization: Positive for smooth muscle cell specific a -actin expression.

Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, and negative for mycoplasma, bacteria, yeast and fungi.
Product Name Product Description Size (vol)

Cat. No.
HPASMC Total Kit, adult

Each kit contains an ampoule of cryopreserved HPASMC (352-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit.

 1 Kit 095352K-05a
 
Cryopreserved HPASMC , adult (>500,000 cells in an ampoule) Cryopreserved at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095352-05a
 
Smooth Muscle Cell Growth Medium

Optimized and ready for use

 500 ml 095311-500
 
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095311K-500
 
Smooth Muscle Cell Basal Medium Add Growth Supplement before use 500 ml 095310-500
 
Smooth Muscle Cell Growth Supplement   30 ml 095311-GS
 
Smooth Muscle Cell Differentiation Medium   250 ml 095311D-250
 
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090K
 
HPASMC RNA , adult

Total RNA prepared from HPASMC

10 µg

25 µg

 095352-R10a

095352-R25a
 
 
  1. Rabinovitch, M., Mossµ Heart Disease in Children, Infants and Adolescents, Williams and Wilkins, Baltimore, p. 856 (1989).
  2. Levine, O.R. et al, J. Pediatr. 83:964 (1973).
  3. Nair, S.S. et al, Arch. Intern. Med. 140:109 (1980).
  4. Rabinovitch, M., Toxicologic Pathology. Vol. 19, No. 4 (Pt.1) pp. 458-468 (1991).
Would you like to have more information?
Contact us