Human Primary Cells
Human Coronary Artery Smooth Muscle Cells (HCASMC)

Human Coronary Artery Smooth Muscle Cells (HCASMC) are derived from the tunica intima and tunica media of normal human, fibrous plaque-free coronary arteries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. Smooth muscle cells are present in various amounts in early fatty streaks and they are the predominant type of cells in fibrous plaques1,2,3. Atherosclerosis of the coronary arteries can cause angina pectoris and heart attack. Intimal smooth muscle proliferation plays a key event in the development of atherosclerosis. HCASMC is an ideal cellular model for the investigations of coronary artery diseases, such as alteration of smooth muscle cell differentiation in atherosclerosis4,5.

  

Human Coronary Artery Smooth Muscle Cells, HCASMC

HCASMC

Actin stained Human Coronary Artery Smooth Muscle Cells, HCASMC

Differentiated HCASMC stained for smooth muscle specific a-actin
Characterization: Positive for smooth muscle cell specific a -actin expression.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name

 Product Description Size (vol)

Cat. No.
HCASMC Total Kit , adult

Each kit contains an ampoule of cryopreserved HAOSMC(350-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit.

 1 Kit 095350K-05a
 
Cryopreserved HCASMC, adult (>500,000 cells in an ampoule) Cryopreserved at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095350-05a
 
Smooth Muscle Cell Growth Medium

Optimized and ready for use.

 500 ml 095311-500
 
Smooth Muscle Cell Growth Mediium Kitl Basal Medium & Growth Supplement packaged separately. Yields 500 ml 095311K-500
 
Smooth Muscle Cell Basal Medium Add Growth Supplement before use. 500 ml 095310-500
 
Smooth Muscle Cell Growth Supplement   30 ml 095311-GS
 
Smooth Muscle Cell Differentiation Medium   250 ml 095311D-250
 
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090K
 
HCASMC RNA , adult

Total RNA prepared from HCASMC.

10 µg

25 µg

 095350-R10a

095350-R25a
 
 
  1. Ross, R.N., Nature 362:801 (1993).
  2. McGill, H.C. Jr., Arteriosclerosis 4:443 (1984).
  3. Jonasson, L. et al, Artherosclerosis 6(2):131 (1986).
  4. Owens. G.K., Atherosclerosis and Coronary Artery Disease Chap3:401 (1996).
  5. Fager, G. et al, In Vitro Cell. Biol. 25(6):511 (1989).
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