Human Primary Cells
Human Pulmonary Artery Endothelial Cells (HPAEC)

 

Human Pulmonary Artery Endothelial Cells (HPAEC) are isolated from normal human pulmonary arteries. They are cryopreserved at second passage and can be cultured and propagated at least 15 population doublings. HPAEC possess an array of enzymatic activities1,2. They respond to a wide range of vasoactive substances3, commensurate with their control of blood pressure and blood pH in vivo . HPAEC have been used for the study of vascular permeability4 and inflammatory responses5. HPAEC in co-culture with HPASMC have been used as a model for pulmonary angiopathy6.

   Human Pulmonary Artery Endothelial Cells
Characterization: Positive for by DiI-Ac-LDL uptake. Positive for factor VIII-related antigen expression.

Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, and negative for mycoplasma, bacteria, yeast and fungi.
Product Name Product Description Size (vol)

Cat. No.
HPAEC Total Kit, adult

Each kit contains an ampoule of cryopreserved HPAEC (302-05a), 500 ml of Endothelial Cell Growth Medium (211-500), and a Subculture Reagent Kit (090K).

 1 Kit 095302K-05a
 
Cryopreserved HPAEC , adult (>500,000 cells in an ampoule) Cryopreserved at second passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095302-05a
 
Endothelial Cell Growth Medium

Optimized and ready for use

 500 ml 095211-500
 
Endothelial Cell Growth Medium Kitl Basal Medium & Growth Supplement packaged separately Yields 500 ml 095211K-500
 
Endothelial Cell Basal Medium Add Growth Supplement before use 500 ml 095210-500
 
Endothelial Cell Growth Supplement   15 ml 095211-GS
 
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090K
 
HPAEC RNA , adult

Total RNA prepared from HPAEC

 10µg 095302-R10a
 
HPAEC RNA , adult

Total RNA prepared from HPAEC

 25µg 095302-R25a
 
 
  1. Ryan, U.S. et al, Tissue Cell 8: 125-146 (1976).
  2. Ryan, U.S. et al, Clin. in Lab. Med. V. 3: 577 (1983).
  3. Ryan, U.S., Handbook of Physiology; The Respiratory System. Am. Physiol. Soc. Chap. 10.:351 (1985).
  4. Downie, G.H. et al, Am. J. Respir. Cell. Mol. Biol. 7:58 (1992).
  5. Farber, H.W. et al, Tissue & Cell 24 (3):355 (1992).
  6. Vender, R.L. et al, In Vitro Cell Dev. Biol. 28A:403 (1992).
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