Human Primary Cells
Human Coronary Artery Endothelial Cells (HCAEC)

Human Coronary Artery Endothelial Cells (HCAEC) are isolated from normal human coronary arteries. They are cryopreserved at second passage and can be cultured and propagated at least 15 population doublings. The endothelium are crucially involved in the regulation of coronary blood flow and cardiac functions, such as thrombosis1,2, atherosclerosis3, and hypertension4. Strategically located between the circulating blood and the vascular smooth muscle, endothelial cells release numerous vasoactive substances regulating the function of vascular smooth muscle and trafficking blood cells5. HCAEC is an ideal candidate for the studies of endothelial cell metabolism and functional vasodilators, such as prostacyclin, bradykinin, nitric oxide and independent of the former endothelium-derived hyperpolarizing factor5. Co-culturing HCAEC with human coronary artery smooth muscle cells (HCASMC) could generate an in vitro model for the cell-cell interaction system3.

  

human coronary artery endothelial cells, HCAEC

HCAEC

LDL uptake human coronary artery endothelial cells, HCAEC

DiI-Ac-LDL Uptake
Characterization: Positive for Factor VIII-related antigen expression. Positive for DiI-Ac-LDL uptake.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name

Product Description

Size (vol)

Cat. No.
HCAEC Total Kit, adult

Each kit contains an ampoule of cryopreserved HCAEC (300-05a), 500 ml of HCAEC Growth Medium (212-500), and a Subculture Reagent Kit (090K).

 1 Kit 095300K-05a
 
Cryopreserved HCAEC, adult (>500,000 cells in an ampoule) Cryopreserved at second passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095300-05a
 
HCAEC Growth Medium

Optimized and ready for use

 500 ml 095212-500
 
HCAEC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095212K-500
 
HCAEC Basal Medium Add Growth Supplement before use 500 ml 095210-500
 
HCAEC Growth Supplement   30 ml 095212-GS
 
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090K
 
HCAEC RNA, adult

HCAEC RNA, adult

Total RNA prepared from HCAEC

Total RNA prepared from HCAEC

 10 µg

25 µg

 095300-R10a

095300-R25a
 
 
  1. Jaffe, E.A., New Engl. J. Med. 296:377 (1997).
  2. Mason, R.G. et al, Arch. Pathol. Lab. Med. 101:61 (1997).
  3. Ross, R.N., Nature 362:801 (1993).
  4. Ryan, U.S., Handbook of Physiology; The Respiratory System. Am. Physiol. Soc. Chap. 10:351 (1985).
  5. Ruschitzka, F.T. et al, Cardiology, 88 Suppl 3:3-19. (1997).
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