Human Primary Cells
Human Carotid Artery Endothelial Cells (HCtAEC)
Human Carotid Artery Endothelial Cells (HCtAEC) are primary endothelial cells isolated from normal carotid arteries and are cryopreserved at third passage. HCtAEC can be propagated at least 10 doublings. The brachiocephalic artery is the first branch from the aortic arch. It bifurcates to form the right common carotid artery, which supplies blood to the head and neck, and the right subclavian artery, which supplies blood to the head and right arm. Both of the corresponding left arteries branch directly from the aortic arch. Carotid artery endothelial cells produce a chemotactic factor for monocytes, but not neutrophils, and this signal is increased after exposure of the endothelial cells to very low-density lipoprotein (b-VLDL) 1. Reduced blood flow during pregnancy is associated with hypotrophy of the endothelial and smooth muscle cells of the carotid artery in the guinea pig2. Oxidative stress suppresses the secretion of endothelin-1 by bovine carotid artery endothelial cells3. Insulin and insulin-like growth factor I stimulate migration and tube forming activity of bovine carotid artery endothelial cells in an angiogenesis in vitro model4, while high glucose-induced serum factor sulfhydryl oxidation, causes growth inhibition5. Matrix metalloproteinases (MMPs) have been implicated in the formation of these plaques in the brachiocephalic artery6, and the carotid artery7.   human carotid artery endothelial cells, hctaec

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name

Product Description

Size (vol)

Cat No.
HCtAEC Total Kit Each kit contains an ampoule of cryopreserved HBcAEC (#3012-05a), 500 ml of HBcAEC Growth Medium (#212-500), and a Subculture Reagent Kit (#090K). 1 kit 0953014K-05a
 
Cryopreserved HCtAEC, adult (>500,000 cells in an ampoule) Cryopreserved in ampoules at second passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 ampoule

 0953014-05a
 
HCtAEC Growth Medium Optimized and ready for use 500 ml 095212-500
 
HCtAEC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500ml 095212K-500
 
Endothelial Cell Basal Medium Add Growth Supplements before use 500 ml 095210-500
 
HCtAEC Growth Supplements   30 ml 095212-GS
 
Subculture Reagent Kit (100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution) 100 ml 095090K
 
Freezing Medium   50ml 095040-50
 
  1. Berliner, JA et al., Arteriosclerosis 6(3):254 (1986).
  2. Jovanovic, S & Jovanovic, A. Hum Reprod. 13(4):1074 (1998).
  3. Saito, T et al., J Cardiovasc Pharmacol. 31(S1):S345 (1998).
  4. Nadao-Hayashi, J et al., Atherosclerosis. 92(2-3):141 (1992).
  5. Nakao-Hayashi, J et al., Atherosclerosis. 97((1):89 (1992).
  6. Johnson, JL et al., Proc Natl Acad Sci USA. 102(43):15575 (2005).
  7. Abilleira, S et al., J Med Genet. 43(12):897 (2006).
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