Human Primary Cells
Human Brachiocephalic Artery Smooth Muscle Cells (HBcASMC)
Human Brachiocephalic Artery Smooth Muscle Cells (HBcASMC) are primary smooth muscle cells isolated from normal brachiocephalic arteries and are cryopreserved at third passage. HASMC can be propagated at least 12 doublings. Human Carotid Artery Smooth Muscle Cells (HCtASMC) are primary smooth muscle cells isolated from normal carotid arteries and are cryopreserved at third passage. HCtASMC can be propagated at least 10 doublings. The brachiocephalic artery is the first branch from the aortic arch. It bifurcates to form the right common carotid artery, which supplies blood to the head and neck, and the right subclavian artery, which supplies blood to the head and right arm. Both of the corresponding left arteries branch directly from the aortic arch. HBcASMC and HCtASMC provide an in vitro system to explore in vivo findings. Apolipoprotein E (apoE) knockout mice serve as a model for atherosclerotic lesion development, as fibrous plaques commonly form in the brachiocephalic artery after 6 months of a high-fat diet, thus providing a small well-defined area in which to study the progressive loss of plaque stability and eventual rupture1,2,3. p53 protects brachiocephalic and aortic smooth muscle cells against apoptosis while promoting apoptosis in macrophages, thus protecting against atherosclerosis development4. Reduced blood flow during pregnancy is associated with hypotrophy of the endothelial and smooth muscle cells of the carotid artery in the guinea pig5.   human carotid artery smooth muscle cells, HCtASMChuman brachiocephalic artery smooth muscle cells, HBcASMC HCtASMC                                                    HBcASMC

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name

Product Description

Size (vol)

Cat No.

SMC Total Kit HBcASMC

HCtASMC

 Each kit contains an ampoule of cryopreserved Smooth Muscle Cells, 500 ml of Smooth Muscle Cell Growth Medium (#311-500), and a Subculture Reagent Kit (#090K). 1 kit 0953512K-05a

0953514K-05a
 

Cryopreserved SMC; adult HBcASMC

HCtASMC

 Cryopreserved in ampoules at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO. >500,000 cells in an ampoule 0953512-05a

0953514-05a
 
Smooth Muscle Cell Growth Medium Optimized and ready for use 500 ml 095311-500
 
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500ml 095311K-500
 
Smooth Muscle Cell Basal Medium Add Growth Supplements before use 500 ml 095310-500
 
Smooth Muscle Cell Growth Supplement   30 ml 095311-GS
 
Smooth Muscle Cell Differentiation Medium   250 ml 095311D-250
 
Smooth Muscle Cell Growth Medium without Antibiotics   500 ml 095311A-500
 
Smooth Muscle Cell Growth Medium without bFGF   500 ml 095311b-500
 
Smooth Muscle Cell Growth Medium without FBS   500 ml 095311F-500
 
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution. 100 ml 095090K
 

  1. Rosenfeld, ME et al., Cur Atheroscler Rep. 4(3):238 (2002).
  2. Johnson, JL & Jackson, CL. Atherosclerosis. 154(2):399 (2001).
  3. Mercer, J et al., Circ Res. 96(6):667 (2005).
  4. Jovanovic, S & Jovanovic, A. Hum Reprod. 13(4):1074 (1998).

 

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