Human Primary Cells
Human Brachiocephalic Artery Endothelial Cells (HBcAEC)

 

Human Brachiocephalic Artery Endothelial Cells (HBcAEC) are primary endothelial cells isolated from normal brachiocephalic arteries and are cryopreserved at third passage. HBcAEC can be propagated at least 12 doublings. The brachiocephalic artery is the first branch from the aortic arch. HBcAEC provide an in vitro system to explore in vivo findings. Apolipoprotein E (apoE) knockout mice serve as a model for atherosclerotic lesion development, as fibrous plaques commonly form in the brachiocephalic artery after 6 months of a high-fat diet, thus providing a small well-defined area in which to study the progressive loss of plaque stability and eventual rupture1,2,3. Matrix metalloproteinases (MMPs) have been implicated in the formation of plaques in the brachiocephalic artery4 and the carotid artery5. In vitro models demonstrate that addition of homocysteine leads to endothelial cell damage6,7, and hyperhomocyteinemia is a risk factor for premature coronary artery, peripheral vascular, and cerebrovascular disease8,9.   Human brachiocephalic artery endothelial cells, HBcAEC

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name

Product Description

Size (vol)

Cat No.
HBcAEC Total Kit

Each kit contains an ampoule of cryopreserved HBcAEC (#3012-05a), 500 ml of HBcAEC Growth Medium (#212-500), and a Subculture Reagent Kit (#090K).

 1 kit 0953012K-05a
Cryopreserved HBcAEC, adult

(>500,000 cells in an ampoule)

Cryopreserved in ampoules at second passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.

1 ampoule

 0953012-05a
HBcAEC Growth Medium

Optimized and ready for use

 500 ml 095212-500
HBcAEC Growth Medium Kit

Basal Medium & Growth Supplement packaged separately

 Yields 500ml 095212K-500
Endothelial Cell Basal Medium

Add Growth Supplements before use

 500 ml 095210-500
HBcAEC Growth Supplements   30 ml 095212-GS
Subculture Reagent Kit

(100 ml each of HBSS, Trypsin/EDTA & Neutralizer Solution)

 100 ml 095090K
Freezing Medium   50ml 095040-50
  1. Williams, H et al., Arterioscler Thromb Vasc Biol. 22(5):788 (2002).
  2. Rosenfeld, ME et al., Cur Atheroscler Rep. 4(3):238 (2002).
  3. Johnson, JL & Jackson, CL. Atherosclerosis. 154(2):399 (2001).
  4. Johnson, JL et al., Proc Natl Acad Sci USA. 102(43):15575 (2005).
  5. Abilleira, S et al., J Med Genet. 43(12):897 (2006).
  6. McCully, KS. Nat Med. 2(4):386 (1996).
  7. Wall, RT et al., Thromb Res. 18(1-2):113 (1980).
  8. Stamler, JS et al., J Clin Invest. 91(1):308 (1993).
  9. Woo, KS et al., Circulation. 96(8):2542 (1997).

 

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