Human Primary Cells
Human Aortic Endothelial Cells (HAOEC)

Human Aortic Endothelial Cells (HAOEC) are primary endothelial cells isolated from normal human aorta. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. They respond to cytokine stimulation by expressing cell adhesion molecules. Aortic endothelial cells have been used as an in vitro model for the study of morphological and ultra-structural changes 1 in the development of atherosclerosis. HAOEC system has also been used for the study of endothelial cell-dependent lymphocyte activation2 and chemoattractant activity for lymphocytes3 in relation to the degree of atherosclerosis.

   Human Aortic Endothelial Cells, HAOEC  
Characterization: Positive for Factor VIII-related antigen expression. Positive for Dil-Ac-LDL uptake.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Pre-Screened HAOEC Cells...

Product Name

Product Description

Size (vol)

Cat. No.
HAOEC Total Kit adult

Each kit contains an ampoule of cryopreserved HAOEC (304-05a), 500 ml of Endothelial Cell Growth Medium (211-500), and a Subculture Reagent Kit.

 1 Kit 095304K-05a
 
Cryopreserved HAOEC adult (>500,000 cells in an ampoule) Cryopreserved at second passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095304-05a
 
Endothelial Cell Growth Medium

Optimized and ready for use

 500 ml 095211-500
 
Endothelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500 ml 095211K-500
 
Endothelial Cell Basal Medium Add Growth Supplement before use 500 ml 095210-500
 
Endothelial Cell Growth Supplement   15 ml 095211-GS
 
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090K
 
HAOEC RNA , adult

Total RNA prepared from HAOEC

 10 µg 25 µg 095304-R10a

095304-R25a
 
 
  1. Antonor, A.S. et al, Atherosclerosis 59:1 (1986).
  2. Ashida, E.R. et al, J. Clin. Invest. 67:1490 (1981).
  3. Farber, H.W. et al, Amer. J. Physiol. 262 (4 parts): H1088 (1992)
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