Human Primary Cells
Human Skeletal Muscle Cells (HSkMC)

Human Skeletal Muscle Cells (HSkMC) are isolated from the limbal skeletal muscle. They are cryopreserved at second passage and can be cultured and propagated for at least 15 population doublings. Human Skeletal Muscle Cells can undergo differentiation to exhibit actin and myosin myofilaments. HSkMC have been used in the study of genetic muscular diseases such as Malignant Hyperthermia1. HSkMC also have the potential to act as a cardiac graft, mending damage to the heart2. Recently, cultured Human Skeletal Muscle cells have been used in micro gravity experiments to study the effects of low gravity environments on Human Skeletal Muscle3.

   Human Skeletal Muscle Cells

Skeletal Muscle Cells

Skeletal Muscle Cells fused to Multinucleated myotube

Characterization: Positive for sarcomere myosin.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Learn about Cell Applications, Inc.'s Pre-Screened Skeletal Muscle Cells...

Product Name

Product Description

Size (vol)

Cat. No.
HSkMC Total Kit, , fetal

Each kit contains an ampoule of cryopreserved HSkMC (150-05f), 500 ml of Skeletal Muscle Cell Growth Medium (151-500), and a Subculture Reagent Kit.

 1 Kit 095150K-05f
Cryopreserved HSkMC , fetal (>500,000 cells in an ampoule) Cryopreserved at second passage in Skeletal Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095150-05f
Skeletal Muscle Cell Growth Medium Optimized and ready for use 500 ml 095151-500
Skeletal Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 095151K-500
Skeletal Muscle Cell Basal Medium Add Growth Supplement before use 500 ml 095150-500
Skeletal Muscle Cell Growth Supplement   50 ml x 2 095151-GS
Skeletal Muscle Cell Differentiation Medium   250 ml 095151D-250
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090K
HSkMC RNA , fetal

Differentiated HSkMC Total RNA , fetal

 Total RNA prepared from HSkMC Multinucleated Myotubes. 10 µg

10 µg

 095150-R10f

095150D-R10f
HSkMC RNA , fetal

Differentiated HSkMC Total RNA , fetal

Total RNA prepared from HSkMC Multinucleated Myotubes.

 25 µg

25 µg

 095150-R25f

095150D-R25f
  1. O’Sullivan, G.H. et al, Biochem Pharmacol 61(12):1479-1485 (2001)
  2. El Oakley, R.M. et al, Ann. Thorac. Surg. 71(5):1724-1733 (2001)
  3. Slentz, D.H. et al, In Vitro Cell. Dev. Biol. Anim. 37(3):148-156 (2001)
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