Human Primary Cells
Human Osteoblasts (HOb)

Human Osteoblasts (HOb) are isolated from normal human bone. They are cryopreserved at second passage and can be cultured and propagated for at least 10 population doublings. HOb sythesizes bone matrix. Matrix mineralization can be induced in the presence of ascorbic acid and B-Glycerophosphate. These cells are commonly used for micro- and macroscopic investigations of the skeletal system, such as the effects of inflammatory mediators,1 the effects of bone morphogenic proteins,2 the physiological control of bone remodeling,3 the regulation of bone metabolism,4 and the study of the chemicals associated with physical trauma.5

   HOb HOb von Kossa Stained
Characterization: Positive for bone mineralization.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol)

Cat. No.
HOb Total Kit adult fetal

Each kit contains an ampoule of cryopreserved HOb (406-05), 500 ml of Osteoblast Growth Medium (417-500), a Subculture Reagent Kit (090K).

 1 Kit 095406K-05a

095406K-05f
Cryopreserved HOb adult fetal (>500,000 cells in an ampoule) Cryopreserved at second passage in Osteoblast Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095406-05a

095406-05f
Osteoblast Growth Medium Optimized and ready for use. 500 ml 095417-500
Osteoblast Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500 ml 095417K-500
Osteoblast Basal Medium Add Growth Supplement before use. 500 ml 095416-500
Osteoblast Growth Supplement   50 ml 095417-GS
Osteoblast Differentiation Medium   250 ml 095417D-250
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 095090K
HOb RNA , fetal

Differentiated HOb RNA , fetal

Stimulated HOb Total RNA , fetal

Total RNA prepared from HOb

 10 µg 095406-R10f

095406D-R10f

095406S-R10f
HOb RNA , fetal

Differentiated HOb RNA , fetal

Stimulated HOb Total RNA , fetal

Total RNA prepared from HOb

 25 µg 095406-R25f

095406D-R25f

095406S-R25f

  1. Takahashi, K., Nippon Yakyurigaku Zasshi. 116(4):232-240 (2000)
  2. Cornish, J. et al Bone 17(4 Suppl):249S-254S (1995)
  3. Malpe, R. et al, J. Bone Miner. Res. 12(3):423-430 (1997)
  4. Reiff. D.A. et al, J. Trauma 50(3):433-438 (2001)

 

 

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