Human Primary Cells - Human Fibroblast-Like Synoviocytes-Osteoarthritis (HFLS-OA)

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Human Primary Cells

Human Fibroblast-Like Synoviocytes-Osteoarthritis (HFLS-OA)

Human Fibroblast-Like Synoviocytes (HFLS) are isolated from synovial tissues obtained from patients with Rheumatoid Arthritis (RA) / Osteoarthritis (OA). They are cryopreserved at second passage and can be cultured and propagated at least 5 population doublings. HFLS are long known for their role in joint destruction by producing cytokines and metalloproteinases that contribute to cartilage degradation^1,2. Proinflammatory cytokines induce the proliferation, collagenase and aggrecanase production and GM-CSF secretion on HFLS^{3, 4}. Ongoing arthritis research also has shown that HFLS express apoptosis and P53 mutations^{5, 6}. We provide two types of HFLS that are useful cellular models for studying the differences between HFLS-RA and HFLS-OA, such as the expression and regulation of proteases⁷ and integrin subunits⁸.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat. No.
HFLS Total Kit normal adult tissue HFLS-OA Total Kit, adult HFLS-RA Total Kit, adult	Each kit contains an ampoule of cryopreserved HFLS (408-05a, 408OA-05a, or 408RA-05a), 500 ml of Synoviocyte Growth Medium (415-500), and a Subculture Reagent Kit (090K).	1 Kit	095408K-05a 095408OAK-05a 095408RAK-05a
Cryopreserved HFLS, normal adult Cryopreserved HFLS-OA , adult Cryopreserved HFLS-RA , adult	(>500,000 cells in an ampoule) Cryopreserved at second passage in Synoviocyte Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	095408-05a 095408OA-05a 095408RA-05a
Synoviocyte Growth Medium	Optimized and ready for use	500 ml	095415-500
Synoviocyte Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	095415K-500
Synoviocyte Basal Medium	Add Growth Supplement before use	500 ml	095414-500
Synoviocyte Growth Supplement		50 ml	095415-GS
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	095090K
HFLS RNA normal adult tissue HFLS-OA RNA , adult HFLS-RA RNA , adult	Total RNA prepared from HFLS Total RNA prepared from HFLS-OA Total RNA prepared from HFLS-RA	10 µg 10 µg 10 µg	095408-R10a 095408OA-R10a 095408RA-R10a
HFLS RNA normal adult tissue HFLS-OA RNA , adult HFLS-RA RNA , adult	Total RNA prepared from HFLS Total RNA prepared from HFLS-OA Total RNA prepared from HFLS-RA	25 µg 25 µg 25 µg	095408-R25a 095408OA-R25a 095408RA-R25a

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