Human Primary Cells
Human Tracheal Epithelial Cells (HTEpC)

Human Bronchial/Tracheal Epithelial Cells (HBEpC/HTEpC) are derived from the surface epithelium of normal human bronchi/trachea. They are cryopreserved at first passage and can be cultured and propagated at least 16 population doublings. Airway epithelial cells are targets in asthma, viral infection, and fibrotic lung disease. These cells have been used to study the regulation of intracellular pH1, the stimulant effect of IL-1b on airway epithelial cell growth2, and the effect of human rhinovirus infection in terms of upregulation of ICAM-1 expression on epithelial cells3.

  
HBEpC

HTEpC

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Product Name Product Description Size (vol)

Cat. No.
HBEpC Total Kit , adult

HTEpC Total Kit, adult

Each kit contains an ampoule of cryopreserved HBEpC/HTEpC (502-05a or 504-05a), 500 ml of Bronchial/Tracheal Epithelial Cell Growth Medium (511-500), and a Subculture Reagent Kit (090K).

 1 Kit 095502K-05a

095504K-05a
Cryopreserved HBEpC, adult

Cryopreserved HTEpC, adult

 (>500,000 cells in an ampoule) Cryopreserved at first passage in Bronchial/Tracheal Epithelial Cell Basal Medium that contains 10% FBS and 10% DMSO. 1 Ampoule 095502-05a

095504-05a
Proliferating Cells Tailored amount of cells at defined passage in customised format (plate, flask, etc)   101SCC_01
HBEpC/HTEpC Growth Medium

Optimized and ready for use.

 500 ml 095511-500
HBEpC/HTEpC Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500 ml 095511K-500
HBEpC/HTEpC Basal Medium Add Growth Supplement before use 500 ml 095510-500
HBEpC/HTEpC Growth Supplement   5 ml 095511-GS
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution. 100 ml 095090K
HBEpC RNA, adult

HTEpC RNA, adult

Total RNA prepared from HBEpC

Total RNA prepared from HTEpC

 10 µg

10 µg

 095502-R10a

095504-R10a
HBEpC RNA, adult

HTEpC RNA, adult

Total RNA prepared from HBEpC

Total RNA prepared from HTEpC

 25 µg

25 µg

 095502-R25a

095504-R25a
  1. Poulsen, J.H. et al, J Physiol.-Abs. Vol. 432 (3) (1996).
  2. Murlas, C.G. et al, Lung 175: 117-126 (1997).
  3. Terajima, M. et al, Lung cellular and molec. physiology, 273 (4):749-759 (1997).

 

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