Transcription factors gene reporter assays
Cignal 10-pathway Reporter Arrays
In a single experiment you can use the Cignal Finder 10-Pathway Reporter Arrays to simultaneously measure the activities of 10 cell signaling pathways known to play critical roles in the biological process being studied in your laboratory. Don't rely on guesswork to predict mechanisms of action. Use the Cignal Finder Arrays to definitively discover important gene functions and pathway interactions.

Which ten pathways?

  • Cancer Reporter Array
Tube format: Cat nr CCA-001 L
Plate Format: Cat nr CCA-101L

Tube/Column Pathway
1 Wnt
2 Notch
3 p53/DNA Damage
4 TGFβ
5 Cell cycle/pRb-E2F
6 NFκB
7 Myc
8 Hypoxia
9 MAPK/ERK
10 MAPK/JNK
11 Cignal negative control
12 Cignal positive control
  • Immune Response Reporter Array
Tube format: Cat nr CCA-002 L
Plate Format: Cat nr CCA-102L

Tube/Column  Pathway
1 NFκB
2 PKC/Ca++
3 Type 1 Interferon
4 Gamma Interferon
5 MAPK/ERK
6 MAPK/JNK
7 TGFβ
8 cAMP/PKA
9 C/EBP
10 Glucocorticoid Receptor
11 Cignal negative control
12 Cignal positive control
  • Development Reporter Array
Tube format: Cat nr CCA-003 L
Plate Format: Cat nr CCA-103L

Tube/Column  Pathway
1 Notch
2 Wnt
3 Myc
4 NFκB
5 TGFβ
6 Cell cycle/pRb-E2F
7 C/EBP
8 cAMP/PKA
9 MAPK/ERK
10 MAPK/JNK
11 Cignal negative control
12 Cignal positive control


How It Works

Each Reporter Array includes 10 Cignal Reporter Assays and two controls in either tube or plate format. All reporter assays are based on dual-luciferase technology. Each reporter consists of a mixture of a pathway-focused transcription factor-responsive firefly luciferase construct and a constitutively expressing Renilla luciferase construct.

Dual-luciferase results are calculated for each transfectant. The change in the activity of each signaling pathway is determined by comparing the normalized luciferase activities of the reporter in treated versus untreated transfectants.

The identically treated negative control serves as a specificity control. The positive control serves as a control for transfection efficiency, by monitoring GFP expression, as well as a positive control for both the firefly and Renilla luciferase assays.

Simple procedure:

  • Transfect Cignal Reporter Assays and test nucleic acids into cells
  • Treat with protein, peptide, or small molecule of interest
  • Perform reporter quantitation using luciferase activity assays