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HDAC inhibitors have shown promise as anti-tumor agents and naturally this has stimulated interest in the screening of compounds for HDAC1 inhibition. Unfortunately, standard techniques for HDAC assay involve the use of [³H]acetyl-histone or [³H]acetyl-histone peptide substrates and a cumbersome acid/ethyl acetate extraction step prior to scintillation counting.

BIOMOL’s HDAC1 Fluorimetric Drug Discovery Kit addresses these problems by providing an assay that can be carried out in two simple mixing steps, all on the same 96-well plate

Includes: Recombinant human HDAC1, Fluor de Lys-“SIRT1” Substrate (p53 379-382) and Developer II, Assay Buffer, trichostatin A (HDAC inhibitor), white and clear 1/2-vol. 96-well plates, detailed instructions.