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Quantibody Human Cytokine Antibody Array 9000

Product name
Quantibody Human Cytokine Antibody Array 9000
Catalog number
QAH-CAA-9000-1 (1-8samplekit)
QAH-CAA-9000-2 (16-22samplekit)
QAH-CAA-9000-4 (32-50samplekit)
Description
Quantibody Human Cytokine Array 9000 Kit. A combination of Human Inflammation Array Q3, Human Growth Factor Array Q1, Human Chemokine Array Q1, Human Receptor Array Q1, Human Cytokine Array Q4, Human Cytokine Array Q5, Human Cytokine Array Q6, Human Cytokine Array Q7, Human Cytokine Array Q8, and Human Cytokine Array Q9. Detects 400 Human Inflammatory Factors, Growth Factors, Chemokines, Receptors, and Cytokines. Suitable for all liquid sample types. 
Product category
Assays & Kits
Product sub category
Multiplex Quantification
Target species
Human
Format
Array
Number of analytes
400
Shipment info
Cool Pack
Source
Raybiotech

Download the datasheet

Download the PDF datasheet

Raybio Quantibody FAQs

1.      What is included in the product?
Our Quantibody product is provided as a kit, which includes all the necessary reagents for performing the experiments. The materials include:

  • Glass Chip with antibody arrays
  • Sample Diluent
  • Lyophilized cytokine standard mix
  • Detection antibody cocktail
  • Streptavidin-Fluorescent dye
  • Wash buffer
  • Manual


2.       What equipment do I need for imaging?
A standard glass slide laser scanner with cy3- or cy5-compatible wavelength is needed for the signal detection. User can choose Fluorescent dye (green, cy3 channel, 555nm excitation, 565nm emission)  signal detection.


3.       What kind of samples can be detected with Quantibody kits? And how much sample do I need for the experiment?
Quantibody arrays can be used in cell culture supernatant, serum, plasma, CSF, tissue lysate, and other body fluid. Typically, 50-100 μl samples are needed for one array.


We recommend the following parameters for your samples: 50-100 μl of cell culture supernatant, 50-100 μl of original or diluted serum or plasma; or 20-200 μg of protein for cell lysates and tissue lysates. In order to minimize the matrix effects and to lower the background of the assay, we recommended that the samples at least diluted 2 fold with Sample Diluent. Dilute the lysate at least 5 fold with Sample Diluent to make a total volume of 50 to 100 μl. For those samples in which you expect a high response for one cytokine and a low response for another, we recommend testing each sample in step dilutions. For example, when testing cell culture supernatant for unknown cytokine concentrations; a 1:2, a 1:10, and a 1:50 dilution are typically used.

4.      What cytokine standards are used in the Quantibody Array?
Laboratories throughout the world use different bioassays and immunoassays to determine cytokine concentrations. As a result, the reported cytokine concentrations in the same sample may vary when using different ELISAs developed by different labs. For this reason, the availability of international standard preparations of cytokines is essential to allow definitive analysis and comparison of results. Quantibody Arrays use either our internal cytokine standards or the International Biological Standards from the National Institute for Biological Standards and Control (NIBSC), the gold cytokine standards.


 5.      Is there any cross-reactivity between the cytokines?
For the available kits, no. . We have tested all of the available antibodies with their specific purified antigens for the cross-reactivity. The antibody pairs used in the kit have been tested to recognize their specific cytokines. There may be some cross-reactivity between antibodies included in two different kits, but analysis of samples containing only a single recombinant cytokine found no cross-reactivity with other antibodies in the same array. For the custom arrays, we will suggest what cytokines can be included in the same array to ensure the proper level of specificity and sensitivity.


6.      Can I save the remaining cytokine standard for next time use?
The cytokine standards in the kit are provided in the lyophilized mixture form to ensure the stability. For the quality purpose, the cytokine standards once reconstituted should be used in a short period of time (2 –3 days) and can’t be refrozen because they may lose activity during the freeze-thaw cycles. For those in need, we suggest only save the highest concentrated vial, and re-do the serial dilution for a second time usage.


7.      Do I need special software to quantify my data?
Yes and no. After imaging by a laser scanner and data extraction with any of the microarray analysis software (GenePix, ScanArray Express, ArrayVision, or MicroVigene), cytokine quantification can be done with virtually any ELISA analysis software. However, our array-specific Q Analyzer software is available to facilitate the data analysis. Instead of tedious calculation, users can now quickly figure out the unknown sample concentration through a simple copy and paste process. The program can automatically remove the outlier spots for greater accuracy.


8.       I would like to try Quantibody products but I don’t have access to a laser scanner. What can I do?
Two options:

  • You may order the Quantibody Arrays and do all the experiments yourselves, and then ship back the slides to tebu-bio. For a certain fee, tebu-bio will do the imaging and all the data analysis for you.
  • Or for quality purpose, we offer a custom processing service. Just sending in your samples to tebu-bio, and we will process the arrays and send back data within 5-10 business days. Service includes: Sample processing with our experienced technicians; Slide imaging & data extraction with our laser scanner and data extraction software; Cytokine quantification with Q Analyzer software; Return results include image and the analysis results in Excel format..
     

Protocol

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